‘A picture paints a thousand words’. However, in the image analysis world, a number is worth a thousand pictures and so accurate image capture is essential if precise scientific measurements (or conclusions) are to be made. Nuclei in the arterial wall Artery adventitia Perivascular nerves Vascular nuclei Fluorescent ligands Fluorescent ligands Endothelial cells Vascular fat & nerves Innervation of PVAT Adipocyte lipid blob Bronchiole Innervation of PVAT Innervation of PVAT Innervation of PVAT Arterial surface Perivascular nerve Adventital fibroblast Arterial smooth muscle Vascular endothelium aortic IEL ridges SMC and EC nuclei Nuclear transfection vascular nerve PVAT innervation Adrenoceptor distribution Vascular SMC Vascular SFP Perfused fixed segment